ID   A4SWE9_POLAQ            Unreviewed;       448 AA.
AC   A4SWE9;
DT   15-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   15-MAY-2007, sequence version 1.
DT   02-SEP-2026, entry version 84.
DE   RecName: Full=N-acetylmuramoyl-L-alanine amidase AmiC {ECO:0000256|ARBA:ARBA00074581};
DE            EC=3.5.1.28 {ECO:0000256|ARBA:ARBA00011901};
GN   OrderedLocusNames=Pnuc_0595 {ECO:0000313|EMBL:ABP33813.1};
OS   Polynucleobacter asymbioticus (strain DSM 18221 / CIP 109841 /
OS   QLW-P1DMWA-1) (Polynucleobacter necessarius subsp. asymbioticus).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC   Burkholderiales; Burkholderiaceae; Polynucleobacter.
OX   NCBI_TaxID=312153 {ECO:0000313|EMBL:ABP33813.1, ECO:0000313|Proteomes:UP000000231};
RN   [1] {ECO:0000313|EMBL:ABP33813.1, ECO:0000313|Proteomes:UP000000231}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 18221 / CIP 109841 / QLW-P1DMWA-1
RC   {ECO:0000313|Proteomes:UP000000231};
RX   PubMed=22675600; DOI=10.4056/sigs.2395367;
RA   Meincke L., Copeland A., Lapidus A., Lucas S., Berry K.W., Del Rio T.G.,
RA   Hammon N., Dalin E., Tice H., Pitluck S., Richardson P., Bruce D.,
RA   Goodwin L., Han C., Tapia R., Detter J.C., Schmutz J., Brettin T.,
RA   Larimer F., Land M., Hauser L., Kyrpides N.C., Ivanova N., Goker M.,
RA   Woyke T., Wu Q.L., Pockl M., Hahn M.W., Klenk H.P.;
RT   "Complete genome sequence of Polynucleobacter necessarius subsp.
RT   asymbioticus type strain (QLW-P1DMWA-1(T)).";
RL   Stand. Genomic Sci. 6:74-83(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes the link between N-acetylmuramoyl residues and L-
CC         amino acid residues in certain cell-wall glycopeptides.; EC=3.5.1.28;
CC         Evidence={ECO:0000256|ARBA:ARBA00001561};
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000256|ARBA:ARBA00004418}.
CC   -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.
CC       {ECO:0000256|ARBA:ARBA00010860}.
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DR   EMBL; CP000655; ABP33813.1; -; Genomic_DNA.
DR   RefSeq; WP_011902438.1; NC_009379.1.
DR   AlphaFoldDB; A4SWE9; -.
DR   GeneID; 31480952; -.
DR   KEGG; pnu:Pnuc_0595; -.
DR   eggNOG; COG0860; Bacteria.
DR   HOGENOM; CLU_014322_2_2_4; -.
DR   Proteomes; UP000000231; Chromosome.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:TreeGrafter.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd02696; MurNAc-LAA; 1.
DR   FunFam; 3.40.630.40:FF:000001; N-acetylmuramoyl-L-alanine amidase; 1.
DR   Gene3D; 2.60.40.3500; -; 1.
DR   Gene3D; 3.40.630.40; Zn-dependent exopeptidases; 1.
DR   InterPro; IPR021731; AMIN_dom.
DR   InterPro; IPR002508; MurNAc-LAA_cat.
DR   InterPro; IPR050695; N-acetylmuramoyl_amidase_3.
DR   PANTHER; PTHR30404; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; 1.
DR   PANTHER; PTHR30404:SF0; N-ACETYLMURAMOYL-L-ALANINE AMIDASE AMIC; 1.
DR   Pfam; PF01520; Amidase_3; 1.
DR   Pfam; PF11741; AMIN; 1.
DR   SMART; SM00646; Ami_3; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:ABP33813.1};
KW   Periplasm {ECO:0000256|ARBA:ARBA00022764};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000231};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          278..433
FT                   /note="MurNAc-LAA"
FT                   /evidence="ECO:0000259|SMART:SM00646"
SQ   SEQUENCE   448 AA;  49278 MW;  C9484FD484F39DFE CRC64;
     MSSKKVNLSK RLTLKTSAQF LSFALLLTEF DIAWGAKILG VRVWPSEDYT RVTLETDKAL
     PITQQILTNP DRLVVDVQGL ELNPTLKDLV AKVKPNDPYI SQVRVGQFQP GVVRLVFDLK
     EPIKPQLFTL DPIGEYNYRM VFDLYPTTPP DPLMELVKSS AKKESALEKS NEEVDLIAQF
     ATNKDKELAK TPAAPVAQAI PETKAPPAAA KYKRLITIAI DPGHGGEDPG AIGAAGSREK
     NVVLSIAKRL KDKIEGEAYM RPYLTRDGDY FVPLHVRVQK ARRVEADLFV SIHADAFIES
     NAKGASVFAL SQMGASSTMA RWMANKENAS DLIGGINIKT QDRQVANLLL DMSTTAQIKD
     SLQVGNSILK QIGGFAPLHK GKVEQASFAV LKAPDIPSIL VETAFISNPQ EEARLNDDGY
     QDRIAEAILK GIKEYFSKNP PVARRINS
//