ID   A4S155_OSTLU            Unreviewed;       380 AA.
AC   A4S155;
DT   15-MAY-2007, integrated into UniProtKB/TrEMBL.
DT   15-MAY-2007, sequence version 1.
DT   02-SEP-2026, entry version 85.
DE   RecName: Full=4-hydroxy-3-methylbut-2-enyl diphosphate reductase {ECO:0000256|ARBA:ARBA00047177};
DE            EC=1.17.7.4 {ECO:0000256|ARBA:ARBA00047177};
GN   Name=IDS {ECO:0000313|EMBL:ABO97595.1};
GN   ORFNames=OSTLU_32979 {ECO:0000313|EMBL:ABO97595.1};
OS   Ostreococcus lucimarinus (strain CCE9901).
OC   Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales;
OC   Bathycoccaceae; Ostreococcus.
OX   NCBI_TaxID=436017 {ECO:0000313|EMBL:ABO97595.1, ECO:0000313|Proteomes:UP000001568};
RN   [1] {ECO:0000313|EMBL:ABO97595.1, ECO:0000313|Proteomes:UP000001568}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCE9901 {ECO:0000313|EMBL:ABO97595.1,
RC   ECO:0000313|Proteomes:UP000001568};
RX   PubMed=17460045; DOI=10.1073/pnas.0611046104;
RA   Palenik B., Grimwood J., Aerts A., Rouze P., Salamov A., Putnam N.,
RA   Dupont C., Jorgensen R., Derelle E., Rombauts S., Zhou K., Otillar R.,
RA   Merchant S.S., Podell S., Gaasterland T., Napoli C., Gendler K.,
RA   Manuell A., Tai V., Vallon O., Piganeau G., Jancek S., Heijde M.,
RA   Jabbari K., Bowler C., Lohr M., Robbens S., Werner G., Dubchak I.,
RA   Pazour G.J., Ren Q., Paulsen I., Delwiche C., Schmutz J., Rokhsar D.,
RA   Van de Peer Y., Moreau H., Grigoriev I.V.;
RT   "The tiny eukaryote Ostreococcus provides genomic insights into the paradox
RT   of plankton speciation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7705-7710(2007).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- PATHWAY: Isoprenoid biosynthesis; dimethylallyl diphosphate
CC       biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-
CC       methylbutenyl diphosphate: step 1/1. {ECO:0000256|ARBA:ARBA00046314}.
CC   -!- PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis
CC       via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-
CC       phosphate: step 6/6. {ECO:0000256|ARBA:ARBA00046313}.
CC   -!- SIMILARITY: Belongs to the IspH family.
CC       {ECO:0000256|ARBA:ARBA00046335}.
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DR   EMBL; CP000588; ABO97595.1; -; Genomic_DNA.
DR   RefSeq; XP_001419302.1; XM_001419265.1.
DR   AlphaFoldDB; A4S155; -.
DR   STRING; 436017.A4S155; -.
DR   GeneID; 5003427; -.
DR   Gramene; ABO97595; ABO97595; OSTLU_32979.
DR   KEGG; olu:OSTLU_32979; -.
DR   eggNOG; ENOG502QPIQ; Eukaryota.
DR   HOGENOM; CLU_027486_4_0_1; -.
DR   OMA; DRIWLTN; -.
DR   OrthoDB; 1698201at2759; -.
DR   Proteomes; UP000001568; Chromosome 8.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:EnsemblPlants.
DR   GO; GO:0046429; F:4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity (ferredoxin); IEA:EnsemblPlants.
DR   GO; GO:0051745; F:4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0050992; P:dimethylallyl diphosphate biosynthetic process; IEA:InterPro.
DR   GO; GO:0019288; P:isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway; IEA:InterPro.
DR   CDD; cd13944; lytB_ispH; 1.
DR   Gene3D; 3.40.50.11270; -; 1.
DR   Gene3D; 3.40.1010.20; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, catalytic domain; 2.
DR   HAMAP; MF_00191; IspH; 1.
DR   InterPro; IPR003451; LytB/IspH.
DR   NCBIfam; TIGR00216; ispH_lytB; 1.
DR   NCBIfam; NF009911; PRK13371.1; 1.
DR   PANTHER; PTHR31619; 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR31619:SF5; 4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTIC; 1.
DR   Pfam; PF02401; LYTB; 1.
PE   3: Inferred from homology;
KW   4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000001568}.
SQ   SEQUENCE   380 AA;  41974 MW;  63E68E5118B06403 CRC64;
     MANDGISYSK TGLVAKMKEN GLSYVEDGIT FKLADAYGFC WGVERAVQMA YEARKQFPEA
     KLWITNEIIH NPTVNDRLSE MGVNFVEETS DGKDFSGVKR GEVVILPAFG ASVHEMKFLA
     DKGANIVDTT CPWVSKVWNA VDTHKRKDFT SIIHGKYSHE ETVATASFAT TYLIVKDMRE
     ANYVRDYILK GGDKAEFMEK FKNAMSQGFD PDEDLDGVGI ANQTTMLKGE TEAIGKLFQS
     TMMEKFGVEN VDQHFVVMDT ICDATQERQD AMYKLVDAKP DIMLVVGGLN SSNTQHLQEI
     SEDKAIPSYW VDTADRLNAD TNSISHKLAH GELVTTDGWL PAGDVVIGIT SGASTPDKVV
     EDVMDVVFAT KRALESAVSR
//