ID   A3LU97_PICST            Unreviewed;       208 AA.
AC   A3LU97;
DT   03-APR-2007, integrated into UniProtKB/TrEMBL.
DT   03-APR-2007, sequence version 1.
DT   02-SEP-2026, entry version 81.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 8 {ECO:0000256|ARBA:ARBA00020637, ECO:0000256|RuleBase:RU364144};
DE   AltName: Full=Mediator complex subunit 8 {ECO:0000256|ARBA:ARBA00031261, ECO:0000256|RuleBase:RU364144};
DE   Flags: Fragment;
GN   Name=MED8 {ECO:0000256|RuleBase:RU364144};
GN   ORFNames=PICST_44472 {ECO:0000313|EMBL:ABN66545.1};
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Pichiomycetes;
OC   Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104 {ECO:0000313|EMBL:ABN66545.1, ECO:0000313|Proteomes:UP000002258};
RN   [1] {ECO:0000313|EMBL:ABN66545.1, ECO:0000313|Proteomes:UP000002258}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545
RC   {ECO:0000313|Proteomes:UP000002258};
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors. {ECO:0000256|RuleBase:RU364144}.
CC   -!- SUBUNIT: Component of the Mediator complex.
CC       {ECO:0000256|RuleBase:RU364144}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC       ECO:0000256|RuleBase:RU364144}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 8 family.
CC       {ECO:0000256|ARBA:ARBA00005716, ECO:0000256|RuleBase:RU364144}.
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DR   EMBL; CP000498; ABN66545.1; -; Genomic_DNA.
DR   RefSeq; XP_001384574.1; XM_001384537.1.
DR   AlphaFoldDB; A3LU97; -.
DR   FunCoup; A3LU97; 148.
DR   STRING; 322104.A3LU97; -.
DR   GeneID; 4838899; -.
DR   KEGG; pic:PICST_44472; -.
DR   eggNOG; ENOG502S8U1; Eukaryota.
DR   HOGENOM; CLU_108151_0_0_1; -.
DR   InParanoid; A3LU97; -.
DR   OMA; PQWYSLQ; -.
DR   OrthoDB; 5329317at2759; -.
DR   Proteomes; UP000002258; Chromosome 4.
DR   GO; GO:0070847; C:core mediator complex; IEA:TreeGrafter.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IEA:TreeGrafter.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   Gene3D; 1.20.58.1710; -; 1.
DR   Gene3D; 6.10.250.2610; -; 1.
DR   InterPro; IPR019364; Mediatior_Med8_fun/met.
DR   PANTHER; PTHR13074; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8; 1.
DR   PANTHER; PTHR13074:SF9; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 8; 1.
DR   Pfam; PF10232; Med8; 1.
PE   3: Inferred from homology;
KW   Activator {ECO:0000256|ARBA:ARBA00023159, ECO:0000256|RuleBase:RU364144};
KW   Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364144};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002258};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163,
KW   ECO:0000256|RuleBase:RU364144};
KW   Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW   ECO:0000256|RuleBase:RU364144}.
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:ABN66545.1"
SQ   SEQUENCE   208 AA;  23974 MW;  322EEA6917317780 CRC64;
     DNSQIPTDSL EAIRNRLNQV HLSLRKLAEQ INLHNRHPYK VKLPSYAHLQ NQFQVLITQL
     NSIATNLSNN DELLRNTNVY PTPLFPTTQQ EGLLTTLLRK KPLPEVDEWM DSALKKVEAE
     RGSSLQKADE MAQWCFAKVQ ELRDEFQFYG FHTVEELDYM ETEEGKKETH EKKQQDRIVD
     DAEARITAGG KKGLSPNQVL KFMCTGVH
//