ID   A3GHB4_PICST            Unreviewed;       377 AA.
AC   A3GHB4;
DT   03-APR-2007, integrated into UniProtKB/TrEMBL.
DT   03-APR-2007, sequence version 1.
DT   04-NOV-2026, entry version 89.
DE   RecName: Full=Sterol 24-C-methyltransferase {ECO:0000256|ARBA:ARBA00018176, ECO:0000256|RuleBase:RU362025};
DE            EC=2.1.1.41 {ECO:0000256|ARBA:ARBA00011968, ECO:0000256|RuleBase:RU362025};
DE   AltName: Full=Delta(24)-sterol C-methyltransferase {ECO:0000256|ARBA:ARBA00075762, ECO:0000256|RuleBase:RU362025};
GN   ORFNames=PICST_80971 {ECO:0000313|EMBL:EAZ63025.1};
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Pichiomycetes;
OC   Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104 {ECO:0000313|EMBL:EAZ63025.1, ECO:0000313|Proteomes:UP000002258};
RN   [1] {ECO:0000313|EMBL:EAZ63025.1, ECO:0000313|Proteomes:UP000002258}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545
RC   {ECO:0000313|Proteomes:UP000002258};
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC       the C-24 of zymosterol to form fecosterol.
CC       {ECO:0000256|RuleBase:RU362025}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=zymosterol + S-adenosyl-L-methionine = fecosterol + S-
CC         adenosyl-L-homocysteine + H(+); Xref=Rhea:RHEA:21128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17038, ChEBI:CHEBI:18252,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789; EC=2.1.1.41;
CC         Evidence={ECO:0000256|RuleBase:RU362025};
CC   -!- PATHWAY: Steroid metabolism; ergosterol biosynthesis; ergosterol from
CC       zymosterol: step 1/5. {ECO:0000256|ARBA:ARBA00005004,
CC       ECO:0000256|RuleBase:RU362025}.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Erg6/SMT family. {ECO:0000256|ARBA:ARBA00038188,
CC       ECO:0000256|PROSITE-ProRule:PRU01022, ECO:0000256|RuleBase:RU362025}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EAZ63025.1}.
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DR   EMBL; AAVQ01000002; EAZ63025.1; -; Genomic_DNA.
DR   AlphaFoldDB; A3GHB4; -.
DR   FunCoup; A3GHB4; 341.
DR   STRING; 322104.A3GHB4; -.
DR   KEGG; pic:PICST_80971; -.
DR   eggNOG; KOG1269; Eukaryota.
DR   HOGENOM; CLU_039068_5_3_1; -.
DR   InParanoid; A3GHB4; -.
DR   OMA; AAEKWEM; -.
DR   OrthoDB; 540004at2759; -.
DR   UniPathway; UPA00768; UER00760.
DR   Proteomes; UP000002258; Chromosome 1.
DR   GO; GO:0005783; C:endoplasmic reticulum; IEA:EnsemblFungi.
DR   GO; GO:0005811; C:lipid droplet; IEA:EnsemblFungi.
DR   GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR   GO; GO:0003838; F:sterol 24-C-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006696; P:ergosterol biosynthetic process; IEA:EnsemblFungi.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   FunFam; 3.40.50.150:FF:000121; Sterol 24-C-methyltransferase; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR050447; Erg6_SMT_methyltransf.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR030384; MeTrfase_SMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR013705; Sterol_MeTrfase_C.
DR   PANTHER; PTHR44068:SF1; HYPOTHETICAL LOC100005854; 1.
DR   PANTHER; PTHR44068; ZGC:194242; 1.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   Pfam; PF08498; Sterol_MT_C; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE   3: Inferred from homology;
KW   Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516,
KW   ECO:0000256|RuleBase:RU362025};
KW   Lipid metabolism {ECO:0000256|ARBA:ARBA00023098,
KW   ECO:0000256|RuleBase:RU362025};
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01022}; Reference proteome {ECO:0000313|Proteomes:UP000002258};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01022};
KW   Steroid biosynthesis {ECO:0000256|ARBA:ARBA00022955,
KW   ECO:0000256|RuleBase:RU362025};
KW   Steroid metabolism {ECO:0000256|ARBA:ARBA00023221,
KW   ECO:0000256|RuleBase:RU362025};
KW   Sterol biosynthesis {ECO:0000256|ARBA:ARBA00023011,
KW   ECO:0000256|RuleBase:RU362025};
KW   Sterol metabolism {ECO:0000256|ARBA:ARBA00023166,
KW   ECO:0000256|RuleBase:RU362025};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01022}.
FT   DOMAIN          77..372
FT                   /note="SAM-dependent methyltransferase Erg6/SMT-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51685"
SQ   SEQUENCE   377 AA;  43323 MW;  0DE5037C73209BE5 CRC64;
     MPDKIELAEK NFARDAKFTE ALHGKSYDKT GLFALTSKAK DASQVAAEGY FKHWNGSVEE
     KDEKSRLTDY SQLTHHYYNL VTDFYEYGWG SSFHFSRYYR GEAFRQATAR HEHFLAHKMN
     INENMKVLDV GCGVGGPGRE ITRFTDCEIV GLNNNDYQIE RANHYAKKYN LDHKLSYVKG
     DFMQMDFEPE TFDAVYAIEA TVHAPVLEGV YSEIYKVLKP GGTFGVYEWV MTDKYDESNE
     EHRKIAYGIE VGDGIPKMYK REVAEQALKN VGFEIEYESD LADRKDEIPW YYPLSGEMKY
     CQTFGDYFTV FRTSKVGRYI TTELVGAMEK IGLAPKGSKQ VTHALEDAAV NLVEGGRQKL
     FTPMMLYVVR KPLDKKN
//