ID   A3GET1_PICST            Unreviewed;       362 AA.
AC   A3GET1;
DT   03-APR-2007, integrated into UniProtKB/TrEMBL.
DT   03-APR-2007, sequence version 1.
DT   02-SEP-2026, entry version 87.
DE   RecName: Full=Mannose-1-phosphate guanyltransferase {ECO:0000256|ARBA:ARBA00018601};
DE            EC=2.7.7.13 {ECO:0000256|ARBA:ARBA00012387};
DE   AltName: Full=ATP-mannose-1-phosphate guanylyltransferase {ECO:0000256|ARBA:ARBA00080455};
DE   AltName: Full=GDP-mannose pyrophosphorylase {ECO:0000256|ARBA:ARBA00031190};
GN   Name=MPG1 {ECO:0000313|EMBL:EAZ63224.1};
GN   ORFNames=PICST_74665 {ECO:0000313|EMBL:EAZ63224.1};
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Pichiomycetes;
OC   Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104 {ECO:0000313|EMBL:EAZ63224.1, ECO:0000313|Proteomes:UP000002258};
RN   [1] {ECO:0000313|EMBL:EAZ63224.1, ECO:0000313|Proteomes:UP000002258}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545
RC   {ECO:0000313|Proteomes:UP000002258};
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Involved in cell wall synthesis where it is required for
CC       glycosylation. Involved in cell cycle progression through cell-size
CC       checkpoint. {ECO:0000256|ARBA:ARBA00024813}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose
CC         + diphosphate; Xref=Rhea:RHEA:15229, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57527,
CC         ChEBI:CHEBI:58409; EC=2.7.7.13;
CC         Evidence={ECO:0000256|ARBA:ARBA00047343};
CC   -!- PATHWAY: Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose
CC       biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP
CC       route): step 1/1. {ECO:0000256|ARBA:ARBA00004823}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC       {ECO:0000256|ARBA:ARBA00007274}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EAZ63224.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAVQ01000001; EAZ63224.1; -; Genomic_DNA.
DR   AlphaFoldDB; A3GET1; -.
DR   FunCoup; A3GET1; 1481.
DR   STRING; 322104.A3GET1; -.
DR   KEGG; pic:PICST_74665; -.
DR   eggNOG; KOG1322; Eukaryota.
DR   HOGENOM; CLU_029499_0_0_1; -.
DR   InParanoid; A3GET1; -.
DR   OMA; GPNCWIC; -.
DR   OrthoDB; 1733332at2759; -.
DR   UniPathway; UPA00126; UER00930.
DR   Proteomes; UP000002258; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR   GO; GO:0004475; F:mannose-1-phosphate guanylyltransferase (GTP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0000032; P:cell wall mannoprotein biosynthetic process; IEA:EnsemblFungi.
DR   GO; GO:0009298; P:GDP-mannose biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd06425; M1P_guanylylT_B_like_N; 1.
DR   FunFam; 2.160.10.10:FF:000017; Mannose-1-phosphate guanyltransferase; 1.
DR   FunFam; 3.90.550.10:FF:000013; mannose-1-phosphate guanyltransferase beta; 1.
DR   Gene3D; 2.160.10.10; Hexapeptide repeat proteins; 1.
DR   Gene3D; 3.90.550.10; Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A; 1.
DR   InterPro; IPR056729; GMPPB_C.
DR   InterPro; IPR045233; GMPPB_N.
DR   InterPro; IPR050486; Mannose-1P_guanyltransferase.
DR   InterPro; IPR005835; NTP_transferase_dom.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   PANTHER; PTHR22572; SUGAR-1-PHOSPHATE GUANYL TRANSFERASE; 1.
DR   Pfam; PF25087; GMPPB_C; 1.
DR   Pfam; PF00483; NTP_transferase; 1.
DR   SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE   3: Inferred from homology;
KW   Cell cycle {ECO:0000256|ARBA:ARBA00023306};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Nucleotidyltransferase {ECO:0000313|EMBL:EAZ63224.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002258};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EAZ63224.1}.
FT   DOMAIN          2..233
FT                   /note="Nucleotidyl transferase"
FT                   /evidence="ECO:0000259|Pfam:PF00483"
FT   DOMAIN          254..361
FT                   /note="Mannose-1-phosphate guanyltransferase C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF25087"
SQ   SEQUENCE   362 AA;  39886 MW;  2C317DCD3E9F8E27 CRC64;
     MKGLILVGGY GTRLRPLTLT LPKPLVEFGN RPMILHQIEA LANAGVTDIV LAVNYRPEVM
     VSTLKQYEEE YGVNITFSVE EEPLGTAGPL KLAEEVLKKD DSPFFVLNSD VICDYPFEEL
     AAFHKAHGGE GTIVATKVDE PSKYGVIVHD RDTPNLIDRF VEKPVEFVGN RINAGLYILN
     PSVIDLIEMK PTSIEKETFP LLVEKNQLYS FDLEGYWMDV GQPKDFLSGT CLYLTSLSKK
     SPEKLSSEKF VHGGNVLIDP TAKIHPSALI GPNVVIGPNV IVGEGARIQR SVLLANSQVK
     DHAWVKSTIV GWNSRIGKWA RTEGVTVLGD DVEVKNEIYV NGAKVLPHKS ISSNVEHEAI
     IM
//