<?xml version="1.0" encoding="UTF-8"  standalone="no" ?>
<uniprot xmlns="http://uniprot.org/uniprot" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://uniprot.org/uniprot http://www.uniprot.org/docs/uniprot.xsd">
<entry dataset="Swiss-Prot" created="2008-01-15" modified="2026-09-02" version="91" xmlns="http://uniprot.org/uniprot">
  <accession>A1V0T9</accession>
  <name>NADK_BURMS</name>
  <protein>
    <recommendedName>
      <fullName evidence="1">NAD kinase</fullName>
      <ecNumber evidence="1">2.7.1.23</ecNumber>
    </recommendedName>
    <alternativeName>
      <fullName evidence="1">ATP-dependent NAD kinase</fullName>
    </alternativeName>
  </protein>
  <gene>
    <name evidence="1" type="primary">nadK</name>
    <name type="ordered locus">BMASAVP1_A0493</name>
  </gene>
  <organism>
    <name type="scientific">Burkholderia mallei (strain SAVP1)</name>
    <dbReference type="NCBI Taxonomy" id="320388"/>
    <lineage>
      <taxon>Bacteria</taxon>
      <taxon>Pseudomonadati</taxon>
      <taxon>Pseudomonadota</taxon>
      <taxon>Betaproteobacteria</taxon>
      <taxon>Burkholderiales</taxon>
      <taxon>Burkholderiaceae</taxon>
      <taxon>Burkholderia</taxon>
      <taxon>pseudomallei group</taxon>
    </lineage>
  </organism>
  <reference key="1">
    <citation type="journal article" date="2010" name="Genome Biol. Evol." volume="2" first="102" last="116">
      <title>Continuing evolution of Burkholderia mallei through genome reduction and large-scale rearrangements.</title>
      <authorList>
        <person name="Losada L."/>
        <person name="Ronning C.M."/>
        <person name="DeShazer D."/>
        <person name="Woods D."/>
        <person name="Fedorova N."/>
        <person name="Kim H.S."/>
        <person name="Shabalina S.A."/>
        <person name="Pearson T.R."/>
        <person name="Brinkac L."/>
        <person name="Tan P."/>
        <person name="Nandi T."/>
        <person name="Crabtree J."/>
        <person name="Badger J."/>
        <person name="Beckstrom-Sternberg S."/>
        <person name="Saqib M."/>
        <person name="Schutzer S.E."/>
        <person name="Keim P."/>
        <person name="Nierman W.C."/>
      </authorList>
      <dbReference type="PubMed" id="20333227"/>
      <dbReference type="DOI" id="10.1093/gbe/evq003"/>
    </citation>
    <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    <source>
      <strain>SAVP1</strain>
    </source>
  </reference>
  <comment type="function">
    <text evidence="1">Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.</text>
  </comment>
  <comment type="catalytic activity">
    <reaction evidence="1">
      <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
      <dbReference type="Rhea" id="RHEA:18629"/>
      <dbReference type="ChEBI" id="CHEBI:15378"/>
      <dbReference type="ChEBI" id="CHEBI:30616"/>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
      <dbReference type="ChEBI" id="CHEBI:58349"/>
      <dbReference type="ChEBI" id="CHEBI:456216"/>
      <dbReference type="EC" id="2.7.1.23"/>
    </reaction>
  </comment>
  <comment type="cofactor">
    <cofactor evidence="1">
      <name>a divalent metal cation</name>
      <dbReference type="ChEBI" id="CHEBI:60240"/>
    </cofactor>
  </comment>
  <comment type="subcellular location">
    <subcellularLocation>
      <location evidence="1">Cytoplasm</location>
    </subcellularLocation>
  </comment>
  <comment type="similarity">
    <text evidence="1">Belongs to the NAD kinase family.</text>
  </comment>
  <dbReference type="EC" id="2.7.1.23" evidence="1"/>
  <dbReference type="EMBL" id="CP000526">
    <property type="protein sequence ID" value="ABM52537.1"/>
    <property type="molecule type" value="Genomic_DNA"/>
  </dbReference>
  <dbReference type="RefSeq" id="WP_004200494.1">
    <property type="nucleotide sequence ID" value="NC_008785.1"/>
  </dbReference>
  <dbReference type="AlphaFoldDB" id="A1V0T9"/>
  <dbReference type="SMR" id="A1V0T9"/>
  <dbReference type="GeneID" id="92980025"/>
  <dbReference type="KEGG" id="bmv:BMASAVP1_A0493"/>
  <dbReference type="HOGENOM" id="CLU_008831_0_1_4"/>
  <dbReference type="GO" id="GO:0005737">
    <property type="term" value="C:cytoplasm"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-SubCell"/>
  </dbReference>
  <dbReference type="GO" id="GO:0005524">
    <property type="term" value="F:ATP binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-ARBA"/>
  </dbReference>
  <dbReference type="GO" id="GO:0046872">
    <property type="term" value="F:metal ion binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0051287">
    <property type="term" value="F:NAD binding"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-ARBA"/>
  </dbReference>
  <dbReference type="GO" id="GO:0003951">
    <property type="term" value="F:NAD+ kinase activity"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="GO" id="GO:0019674">
    <property type="term" value="P:NAD+ metabolic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="InterPro"/>
  </dbReference>
  <dbReference type="GO" id="GO:0006741">
    <property type="term" value="P:NADP+ biosynthetic process"/>
    <property type="evidence" value="ECO:0000501"/>
    <property type="project" value="UniProtKB-UniRule"/>
  </dbReference>
  <dbReference type="Gene3D" id="3.40.50.10330">
    <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Gene3D" id="2.60.200.30">
    <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="HAMAP" id="MF_00361">
    <property type="entry name" value="NAD_kinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR017438">
    <property type="entry name" value="ATP-NAD_kinase_N"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR017437">
    <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR016064">
    <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
  </dbReference>
  <dbReference type="InterPro" id="IPR002504">
    <property type="entry name" value="NADK"/>
  </dbReference>
  <dbReference type="NCBIfam" id="NF002561">
    <property type="entry name" value="PRK02155.1"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR20275">
    <property type="entry name" value="NAD KINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="PANTHER" id="PTHR20275:SF0">
    <property type="entry name" value="NAD KINASE"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF01513">
    <property type="entry name" value="NAD_kinase"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="Pfam" id="PF20143">
    <property type="entry name" value="NAD_kinase_C"/>
    <property type="match status" value="1"/>
  </dbReference>
  <dbReference type="SUPFAM" id="SSF111331">
    <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
    <property type="match status" value="1"/>
  </dbReference>
  <proteinExistence type="inferred from homology"/>
  <keyword id="KW-0067">ATP-binding</keyword>
  <keyword id="KW-0963">Cytoplasm</keyword>
  <keyword id="KW-0418">Kinase</keyword>
  <keyword id="KW-0520">NAD</keyword>
  <keyword id="KW-0521">NADP</keyword>
  <keyword id="KW-0547">Nucleotide-binding</keyword>
  <keyword id="KW-0808">Transferase</keyword>
  <feature type="chain" id="PRO_1000005394" description="NAD kinase">
    <location>
      <begin position="1"/>
      <end position="300"/>
    </location>
  </feature>
  <feature type="active site" description="Proton acceptor" evidence="1">
    <location>
      <position position="75"/>
    </location>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="75"/>
      <end position="76"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="149"/>
      <end position="150"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="177"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="179"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <begin position="190"/>
      <end position="195"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="214"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <feature type="binding site" evidence="1">
    <location>
      <position position="248"/>
    </location>
    <ligand>
      <name>NAD(+)</name>
      <dbReference type="ChEBI" id="CHEBI:57540"/>
    </ligand>
  </feature>
  <evidence type="ECO:0000255" key="1">
    <source>
      <dbReference type="HAMAP-Rule" id="MF_00361"/>
    </source>
  </evidence>
  <sequence length="300" mass="32380" checksum="F74C09919CEECEEA" modified="2007-02-06" version="1">MKIGHQFHTVALVGRSNTPGIAEPLASLAACIAKRGFEVVFEADTAQAIGSAGYPALTPAEIGARAGVAVVLGGDGTMLGMGRQLAPYKTPLIGINHGRLGFITDIPASDMREVVPMMLAGSYEREERTLLEARIVRNGEPIYHALAFNDVVVNRSGFSGMAELRVSVDGRFMYNQRSDGLIVATPTGSTAYALSSQGPILHPQLQGIVLVPIAPHALSNRPIVLPDDSKIAIQIIGGRDVNVNFDMQSFTALELNDTIEVRRSKHTVPFLHPVGYSYYATLRKKLHWNEHPSSEEDDDA</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>