ID   RLMF_PSYIN              Reviewed;         310 AA.
AC   A1SSI8;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   18-JUN-2025, entry version 88.
DE   RecName: Full=Ribosomal RNA large subunit methyltransferase F {ECO:0000255|HAMAP-Rule:MF_01848};
DE            EC=2.1.1.181 {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=23S rRNA mA1618 methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
DE   AltName: Full=rRNA adenine N-6-methyltransferase {ECO:0000255|HAMAP-Rule:MF_01848};
GN   Name=rlmF {ECO:0000255|HAMAP-Rule:MF_01848};
GN   OrderedLocusNames=Ping_0599;
OS   Psychromonas ingrahamii (strain DSM 17664 / CCUG 51855 / 37).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Alteromonadales; Psychromonadaceae; Psychromonas.
OX   NCBI_TaxID=357804;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17664 / CCUG 51855 / 37;
RX   PubMed=18460197; DOI=10.1186/1471-2164-9-210;
RA   Riley M., Staley J.T., Danchin A., Wang T.Z., Brettin T.S., Hauser L.J.,
RA   Land M.L., Thompson L.S.;
RT   "Genomics of an extreme psychrophile, Psychromonas ingrahamii.";
RL   BMC Genomics 9:210-210(2008).
CC   -!- FUNCTION: Specifically methylates the adenine in position 1618 of 23S
CC       rRNA. {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=adenosine(1618) in 23S rRNA + S-adenosyl-L-methionine = N(6)-
CC         methyladenosine(1618) in 23S rRNA + S-adenosyl-L-homocysteine + H(+);
CC         Xref=Rhea:RHEA:16497, Rhea:RHEA-COMP:10229, Rhea:RHEA-COMP:10231,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:74411, ChEBI:CHEBI:74449; EC=2.1.1.181;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01848};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01848}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. METTL16/RlmF
CC       family. {ECO:0000255|HAMAP-Rule:MF_01848}.
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DR   EMBL; CP000510; ABM02453.1; -; Genomic_DNA.
DR   RefSeq; WP_011769012.1; NC_008709.1.
DR   AlphaFoldDB; A1SSI8; -.
DR   SMR; A1SSI8; -.
DR   STRING; 357804.Ping_0599; -.
DR   KEGG; pin:Ping_0599; -.
DR   eggNOG; COG3129; Bacteria.
DR   HOGENOM; CLU_027534_3_0_6; -.
DR   OrthoDB; 1115728at2; -.
DR   Proteomes; UP000000639; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0052907; F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0070475; P:rRNA base methylation; IEA:TreeGrafter.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   HAMAP; MF_01848; 23SrRNA_methyltr_F; 1.
DR   InterPro; IPR010286; METTL16/RlmF.
DR   InterPro; IPR016909; rRNA_lsu_MeTfrase_F.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; NF008725; PRK11727.1; 1.
DR   PANTHER; PTHR13393:SF0; RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16; 1.
DR   PANTHER; PTHR13393; SAM-DEPENDENT METHYLTRANSFERASE; 1.
DR   Pfam; PF05971; Methyltransf_10; 1.
DR   PIRSF; PIRSF029038; Mtase_YbiN_prd; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Methyltransferase; Reference proteome; rRNA processing;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..310
FT                   /note="Ribosomal RNA large subunit methyltransferase F"
FT                   /id="PRO_0000349943"
SQ   SEQUENCE   310 AA;  34853 MW;  16FE810B6AD4BCFC CRC64;
     MTKDIQVKKL LHPRNLHRGH YDLKQLCEQS PLLSTFLRTN PKGEQTLDFA EPQAVLLLNQ
     VLLKQFYHVD FWQIPKGYLC PPIPGRVDYI HYLADLLGDT FHGKIPEGKQ VKVLDIGTGA
     NCIYPILGSQ SYGWSFVGTD IDPLSVKMAG LIIKSNVSLK PFIKVQLQAN KQAIFAGIIK
     PKDKFTLTMC NPPFHASMEK ALAGSARKIK NLSNDQNNLS RVLNFAGQEG ELCCAGGEIR
     FLKQMIQESK DYARQVCWFT SLVSKSDNIA PLKQQLEKVG AEHVKVIKMA QGQKVSRFIA
     WSFLPQPQNF
//