ID   A0ALP4_LISW6            Unreviewed;       604 AA.
AC   A0ALP4;
DT   28-NOV-2006, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2006, sequence version 1.
DT   02-SEP-2026, entry version 92.
DE   SubName: Full=N-acetylmuramoyl-L-alanine amidase, family 2 {ECO:0000313|EMBL:CAK21926.1};
DE            EC=3.5.1.28 {ECO:0000313|EMBL:CAK21926.1};
GN   Name=ami {ECO:0000313|EMBL:CAK21926.1};
GN   OrderedLocusNames=lwe2508 {ECO:0000313|EMBL:CAK21926.1};
OS   Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / CCUG 15529
OS   / CIP 8149 / NCTC 11857 / SLCC 5334 / V8).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Listeriaceae;
OC   Listeria.
OX   NCBI_TaxID=386043 {ECO:0000313|EMBL:CAK21926.1, ECO:0000313|Proteomes:UP000000779};
RN   [1] {ECO:0000313|EMBL:CAK21926.1, ECO:0000313|Proteomes:UP000000779}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35897 / DSM 20650 / CCUG 15529 / CIP 8149 / NCTC 11857 /
RC   SLCC 5334 / V8 {ECO:0000313|Proteomes:UP000000779};
RX   PubMed=16936040; DOI=10.1128/JB.00758-06;
RA   Hain T., Steinweg C., Kuenne C.T., Billion A., Ghai R., Chatterjee S.S.,
RA   Domann E., Kaerst U., Goesmann A., Bekel T., Bartels D., Kaiser O.,
RA   Meyer F., Puehler A., Weisshaar B., Wehland J., Liang C., Dandekar T.,
RA   Lampidis R., Kreft J., Goebel W., Chakraborty T.;
RT   "Whole-genome sequence of Listeria welshimeri reveals common steps in
RT   genome reduction with Listeria innocua as compared to Listeria
RT   monocytogenes.";
RL   J. Bacteriol. 188:7405-7415(2006).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the N-acetylmuramoyl-
CC       L-alanine amidase 2 family. {ECO:0000256|ARBA:ARBA00006088}.
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DR   EMBL; AM263198; CAK21926.1; -; Genomic_DNA.
DR   RefSeq; WP_011703235.1; NC_008555.1.
DR   AlphaFoldDB; A0ALP4; -.
DR   STRING; 386043.lwe2508; -.
DR   PaxDb; 386043-lwe2508; -.
DR   GeneID; 61190427; -.
DR   KEGG; lwe:lwe2508; -.
DR   eggNOG; COG5632; Bacteria.
DR   HOGENOM; CLU_014518_0_0_9; -.
DR   OrthoDB; 9816557at2; -.
DR   Proteomes; UP000000779; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008745; F:N-acetylmuramoyl-L-alanine amidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009253; P:peptidoglycan catabolic process; IEA:InterPro.
DR   CDD; cd06583; PGRP; 1.
DR   FunFam; 3.40.80.10:FF:000013; Autolysin amidase; 1.
DR   FunFam; 2.30.30.170:FF:000001; Internalin B; 2.
DR   Gene3D; 2.30.30.170; -; 4.
DR   Gene3D; 3.40.80.10; Peptidoglycan recognition protein-like; 1.
DR   InterPro; IPR036505; Amidase/PGRP_sf.
DR   InterPro; IPR002502; Amidase_domain.
DR   InterPro; IPR051056; Glycosyl_Hydrolase_73.
DR   InterPro; IPR025987; GW_dom.
DR   InterPro; IPR038200; GW_dom_sf.
DR   NCBIfam; NF033202; GW_glycos_SH3; 4.
DR   PANTHER; PTHR33308:SF10; EXO-GLUCOSAMINIDASE LYTG; 1.
DR   PANTHER; PTHR33308; PEPTIDOGLYCAN HYDROLASE FLGJ; 1.
DR   Pfam; PF01510; Amidase_2; 1.
DR   Pfam; PF13457; GW; 4.
DR   SMART; SM00644; Ami_2; 1.
DR   SUPFAM; SSF55846; N-acetylmuramoyl-L-alanine amidase-like; 1.
DR   SUPFAM; SSF82057; Prokaryotic SH3-related domain; 4.
DR   PROSITE; PS51780; GW; 4.
PE   3: Inferred from homology;
KW   Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CAK21926.1};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000779};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..604
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5039177333"
FT   DOMAIN          281..359
FT                   /note="GW"
FT                   /evidence="ECO:0000259|PROSITE:PS51780"
FT   DOMAIN          362..440
FT                   /note="GW"
FT                   /evidence="ECO:0000259|PROSITE:PS51780"
FT   DOMAIN          446..524
FT                   /note="GW"
FT                   /evidence="ECO:0000259|PROSITE:PS51780"
FT   DOMAIN          527..604
FT                   /note="GW"
FT                   /evidence="ECO:0000259|PROSITE:PS51780"
SQ   SEQUENCE   604 AA;  67793 MW;  AE12A665DB58EA5C CRC64;
     MKKLVKSAVV FASLAFIGAS ATMITENASA ASIDTVQKVD DQSIYIPKGI RDGTATEEHD
     GFEDETNSVL KEVPLLRAAG YPDVNSYIKT NKFSTASIEK QLKSQFPKFN YRNGYGKPEG
     IVIHETANNS STITGEINYM STNYNNAFVH AFVDKSRIIQ IHPTENGVWG AGQYANARFI
     QVELVRSKTF DEFSRSINNY AYYTAYLLDQ YKLPVDSAHS DGKGTVWSHD AVTRYLGGTT
     HTDPVGYFNQ WGYNFNSFVT LVNEKYKAIQ SSKVTYDKIE YDKGVTAYAR VKTAPGNAVW
     TKPYKTEGAK GINPLSVYVG KNMRILREAK TPITTWYQFS IDGKTIGWVD TRALDTFYKQ
     SMEKPANLTR YVLANKANEA YYKVPVADSA VKWGTLSTYK NQKLTVDRQA TIEGQLWYRI
     KTSSTFIGWT KAANLTTVSP VDKIEYDKGV TAYARVKTAP GNAVWTKPYK TEGAKGVNPL
     SVYVGKNMRI LREAKTPITT WYQFSIDGKT IGWVDTRALN TFYKQSMEKP ANLTRYVLAN
     KANEAYYKVP VADSAVKWGT LSTYKNQKLT VDRQATIEGQ LWYRIKTSST FIGWTKAANL
     STSK
//